Senior Lecturer in Biopharmaceutical Science

I am a biochemist and molecular biologist specilising in protein engineering and non-coding RNA biology. In 2013, after almost 12 years of being associated with the biopharmaceutical industry, I decided to join the University of Sunderland as a Senior Lecturer in Biopharmaceutical Science. 

Teaching and supervision


Research interests for potential research students

  • Protein biochemistry and engineering
  • Biotechnology
  • RNA biology
  • Cellular regeneration
  • Embryogenesis

Research

My research is crudely spit into the following areas:

Biotherapeutics: We are interested in all aspects of biotherapeutic development, from recombinant proteins to siRNAs and gene therapies.

Bioprocessing: The expression and purification steps employed for the manufacture of biotherapeutics require optimisation and development. We are interested in the profiling of these steps through the use of DoE and statistical analysis techniques.

Bioinformatics: We employ bioinformatics and molecular modelling in the design and development of new therapeutics.

Cheminformatics: The application of computational methods for mining and modelling of chemical datasets is a developing research area for us. We are using publicly available datasets for the identification of established drugs for re-purposing in other therapeutic areas.

Regenerative medicine: Through the use of planarian worms we are profiling regenerative pathways with the ultimate goal of being able to develop and test regeneration-modulating biotherapeutics.

Publications

Number of items: 11.

Article

Abu Abed, O. S., Elkordy, Amal, Chaw, Cheng, Williams, Lee and Carlile, Mark (2016) Development and Characterisation of Polymeric Nanacapsules Containing Macromolecule. Molecular Therapy, 24 (Supp 1). S237-S238. ISSN 1525-0016

Werner, Andreas, Cockell, Simon, Falconer, Jane, Carlile, Mark, Alnumeir, Sammer and Robinson, John (2014) Contribution of natural antisense transcription to an endogenous siRNA signature in human cells. BMC Genomics., 15 (19). pp. 1471-2164. ISSN 1471-2164

Falconer, Jane, Werner, A, Cockell, S, Carlile, Mark, Alnumeir, Sammer and Robinson, JH (2014) Contribution of natural antisense transcription to an endogenous siRNA signature in human cells. BMC Genomics, 15 (19). ISSN 1471-2164

Carlile, Mark, Swan, D, Jackson, K, Preston-Fayers, K, Flicek, P and Werner, A (2009) Strand selective generation of endo-siRNAs from the Na/phosphate transporter gene Slc34a1 in murine tissues. Nucleic Acids Research, 37 (7). pp. 2274-2282. ISSN 0305-1048

Werner, A, Carlile, Mark and Swan, D (2009) What do natural antisense transcripts regulate? RNA Biology, 6 (1). pp. 43-48. ISSN 1547-6286

Carlile, Mark, Nalbant, P, Preston-Fayers, K, McHaffie, G S and Werner, A (2008) Processing of naturally occurring sense/antisense transcripts of the vertebrate Slc34a gene into short RNAs. Physiological Genomics, 34 (1). pp. 95-100. ISSN 1094-8341

Werner, A, Schmutzler, G, Carlile, Mark, Miles, C G and Peters, H (2007) Expression profiling of antisense transcripts on DNA arrays. Physiological Genomics, 28 (3). pp. 294-300. ISSN 1094-8341

Book Section

Carlile, Mark and Werner, Andreas (2014) Extraction and Non-radioactive Detection of Small RNA Molecules. In: Animal Endo-SiRNAs. Methods in Molecular Biology, 1173 (186). Springer, UK. ISBN 978-1-4939-0930-8

Conference or Workshop Item

Amer, Amer H., Anderson, Rosaleen, Carlile, Mark, Dawson, Peter and Ashton, Mark (2017) A 3D-QSAR Pharmacophore based virtual screening and docking approach to identify novel HIV-1 entry inhibitors. In: New Approaches in Medicinal Chemistry II, 01 Feb 2017, Eli Lilly, Windlesham, UK. (Unpublished)

Abu Abed, O. S., Chaw, Cheng, Carlile, Mark, Williams, Lee and Elkordy, Amal (2015) Effects of copolymer type and excipient on the size and encapsulation efficiency of lysozyme containing nanocapsules. In: 6th APS International PharmSci 2015, Innovation in Pharmaceutical Sciences, 7 - 9 Sept 2015, East Midlands Conference Centre, Nottingham, UK. (Unpublished)

Abu Abed, O. S., Chaw, Cheng, Carlile, Mark and Elkordy, Amal (2015) Evaluating the effect of pH, buffer conditions, and excipients on conformational stability of trypsin. In: 50th Anniversary Conference of the RSC Thermal Methods Group, 30 Mar - 1 Apr 2015, Churchill College, Cambridge, UK. (Unpublished)

This list was generated on Thu Sep 19 16:04:01 2019 BST.
  • Protein biochemistry
  • RNA biology
  • Biotechnology (recombinant technologies)
  • Drug development and manufacture
Personal web profile: https://mcarlilelab.tumblr.com/

Last updated 11 June 2019